Student Solution

-->

"Education is the most powerful weapon which you can use to change the world”
– Nelson Mandela

1 University

1 Course

2 Subjects

Group Activity - Gram Stain

Group Activity - Gram Stain

Q 1. Give the Gram stain result of each of the three panels shown below. Remember, Gram stain results must include whether Gram negative or positive, the shape and arrangement.2. indicate which panel likely fits the following; 3. What are the four main steps of Gram staining? List the four steps and indicate the role of each step.4. Peter was given a mixed culture containing one Gram positive and one Gram negative to perform a Gram stain. When the staining was done, looking through the oil immersion lens, he saw that all bacteria on the slide were purple. Give one reason why Peter only saw purple bacteria.5. What is the total magnification of the image Peter saw under the oil immersion lens?

View Related Questions

Solution Preview

1.A is Gram positive , cocci and is arranged in clusters, B is Gram Negative , bacilli and is arranged in clusters, C is a mix of Gram positive bacteria and Gram negative bacteria , cocci and bacteria are in groups of 2-5. 2.Staphylococcus aureus - A Mixed culture - C E. coli - B 3.1. Primary stain - role= initial dye 2. Addition of mordant - role= help stain bind 3. Decolorizing - role= identifies bacteria , crystal violet is removed 4. Counter stain -role= shows results , pink = Gram negative purple= Gram positive